Long-term stability of serum samples positive for carbohydrate deficient transferrin (CDT) routinely stored at -20 °C.
Author(s): Veronesi A, Rota C, Carone C, Trenti T, Cariani E
Publication: Clin Chem Lab Med, 2016, Vol. 54, Page e285-7
PubMed ID: 26974144 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of frozen storage at -20°C on carbohydrate deficient transferrin (CDT) levels in serum.
Conclusion of Paper
Median levels of CDT decreased after 1 year of frozen storage but the difference was significant only after 3 years. The percentage of specimens that remained positive (CDT value ≥2.0%) was 85% after 1 or 2 years of storage and 75% after 3 years of storage.
Studies
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Study Purpose
This study investigated the effects of frozen storage at -20°C on carbohydrate deficient transferrin (CDT) levels in serum. Serum collected by an unspecified method from March-June in 2012, 2013, and 2014 and determined to be positive for CDT at the time of blood draw (≥2.0% cut-off value) was stored frozen in the original tube for 1 (n=48), 2 (n=48), or 3 years (n=24) and not thawed until re-analysis of CDT levels by capillary electrophoresis.
Summary of Findings:
Median levels of CDT decreased after 1 year (-4.8%) and 2 years of frozen storage (-7.0%) but the difference was significant only after 3 years (-9.1%, P=0.032). The percentage of specimens remaining positive (CDT value ≥2.0%) was 85% after 1 or 2 years of storage and 75% after 3 years of storage.
Biospecimens
Preservative Types
- None (Fresh)
- Frozen
Diagnoses:
- Other diagnoses
Platform:
Analyte Technology Platform Protein Capillary electrophoresis-MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 1 year
2 years
3 years