The addition of MESNA in vitro prolongs prothrombin time similar to N-acetyl cysteine.

Author(s): Blasi A, Caballo C, Galán AM, Taura P, Beltran J, Escolar G, Lozano M

Publication: Clin Chem Lab Med, 2013, Vol. 51, Page e297-9

PubMed ID: 23929884 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of the adjuvant drug, MESNA, on PT, aPTT, and thromboelastometry in spiked blood specimens. Blood was incubated with either saline, 1000 uM of MESNA, or 2000 uM of MESNA for 1 hour at 37 degrees C prior to analysis.

   Summary of Findings:

   When blood specimens were incubated with 1000 uM of MESNA, PT ratio increased significantly (p<0.01), and MCF decreased slightly but significantly (p<0.01) compared to specimens incubated with saline (baseline). When blood specimens were incubated with 2000 uM of MESNA, PT ratio was significantly increased from baseline but was not significantly different from that observed in specimens incubated with 1000 uM MESNA. Interestingly, MCF was unaffected by incubation of blood with 2000 uM MESNA compared to blood incubated with saline. No significant changes were observed in aPTT, CT, CFT, alpha angle, or clot lysis parameters when blood was incubated with any amount of MESNA.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen components	Saline 1000 uM MESNA 2000 uM MESNA

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