Advancing haemostasis automation--successful implementation of robotic centrifugation and sample processing in a tertiary service hospital.

Author(s): Sédille-Mostafaie N, Engler H, Lutz S, Korte W

Publication: Clin Chem Lab Med, 2013, Vol. 51, Page 1273-8

PubMed ID: 23241682 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of centrifuge speed and duration on coagulation and hematological parameters in automated and manually processed plasma. Specimens were centrifuged automatically at 3000 x g for 7 min or manually at 1500 x g for 5 min or 20 min.  Sodium citrate blood was processed within 2-3 h of collection.

   Summary of Findings:

   Platelet counts decreased from 17,000-125,000 platelets/uL in specimens centrifuged manually at 1500 x g for 5 min, to 3000-9000 platelets/uL in plasma after automated centrifugation at 3000 x g for 7 min and to 1000-6000 platelets/uL in plasma processed manually at 1500 x g for 20 min. Thus the plasma obtained by the automated centrifugation or centrifugation at 1500 x g for 20 min was classified as platelet poor plasma while the plasma obtained by manual centrifugation at 1500 x g for 5 min was not. When specimens were processed with automated centrifugation at 3000 x g for 7 min, levels of fibrinogen, FVIII, FIX, FXI, FXII and D-dimer, partial thromboplastin time (PTT), the international normalized ratio (INR), and quick activated partial thromboplastin time(aPTT) were very strongly correlated (R2=0.964-0.987), FV levels were strongly correlated (R2=0.888), and FVII and FX were modestly correlated (R2=0.586-0.685) with those in plasma obtained by manual centrifugation at 1500 x g for 5 min. Very strong correlations in levels of FV, FVIII, FIX, FXI, and FXII (R2=0.916-0.980) and strong correlations in levels of FVII and FX (R2=0.777-0.822) were observed between plasma processed by automated centrifugation at 3000 x g for 7 min and plasma obtained by manual centrifugation at 1500 x g for 20 min. Levels of FV, FVII, FVIII, FIX, FXI and FXII were very strongly correlated (R2=0.917-0.986) and levels of FX were strongly correlated (R2=0.792) between plasma obtained by manual centrifugation at 1500 x g for 5 min and 30 min.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer
   Morphology	Hematology/ auto analyzer
   Peptide	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Platelet-poor plasma
   Biospecimen Aliquots and Components	Blood processing method	AutoMate 800 preanalytical sample processor Manual processing
   Biospecimen Aliquots and Components	Centrifugation	Automated Manual Multiple durations compared Multiple speeds compared

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