Reference intervals of extended erythrocyte and reticulocyte parameters.

Author(s): Hoffmann JJ, van den Broek NM, Curvers J

Publication: Clin Chem Lab Med, 2012, Vol. 50, Page 941-8

PubMed ID: 22628338 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of patient gender and age and delayed analysis on erythrocyte and reticulocyte parameters. Blood was collected into K2EDTA tubes.

   Summary of Findings:

   RBC, Hb and reticulocyte counts were unaffected by storage of blood specimens at room temperature, but MCV increased with overnight storage at room temperature (p<0.0001), and consequently, increases in hypochromic RBC, macrocytic RBC, and MCVr, as well as decreases in MCHC and MCHCr were observed. WBC viability was stable for at least 6 h at room temperature, but then decreased significantly with longer storage durations (p<0.001). The authors report that while absolute reticulocyte counts were not significantly different between specimens from males and females, mean Hb and RBC and the percentage of reticulocytes were higher in females than males (p<0.001, all). MCV, MCVr, macrocytic RBC, RDW, MCH and MCHr tended to increase with patient age, and MCHC and MCHCr tended to decrease with patient age (p<0.0001, all). Reticulocytes were lower in specimens from patients younger than 18 years old compared to counts in specimens from patients who were 18 years old or older.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient gender	Female Male
   Preaquisition	Patient age	0-5 years 6-10 years 11-15 years 16-20 years 21-25 years 26-30 years 31-35 years 36-40 years 41-45 years 46-50 years 51-55 years 56-60 years 61-65 years 66-70 years 71-75 years 75-80 years 81-85 years 86-90 years 90-95 years 96-100 years
   Storage	Time at room temperature	0 h 2 h 4 h 6 h 8 h 10 h 12 h 14 h 16 h 18 h 20 h 22 h 24 h 28 h 32 h 36 h 40 h 44 h 48 h

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