Reference intervals of extended erythrocyte and reticulocyte parameters.
Author(s): Hoffmann JJ, van den Broek NM, Curvers J
Publication: Clin Chem Lab Med, 2012, Vol. 50, Page 941-8
PubMed ID: 22628338 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of patient gender and age and delayed analysis on erythrocyte and reticulocyte parameters. Blood was collected into K2EDTA tubes.
Summary of Findings:
RBC, Hb and reticulocyte counts were unaffected by storage of blood specimens at room temperature, but MCV increased with overnight storage at room temperature (p<0.0001), and consequently, increases in hypochromic RBC, macrocytic RBC, and MCVr, as well as decreases in MCHC and MCHCr were observed. WBC viability was stable for at least 6 h at room temperature, but then decreased significantly with longer storage durations (p<0.001). The authors report that while absolute reticulocyte counts were not significantly different between specimens from males and females, mean Hb and RBC and the percentage of reticulocytes were higher in females than males (p<0.001, all). MCV, MCVr, macrocytic RBC, RDW, MCH and MCHr tended to increase with patient age, and MCHC and MCHCr tended to decrease with patient age (p<0.0001, all). Reticulocytes were lower in specimens from patients younger than 18 years old compared to counts in specimens from patients who were 18 years old or older.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Patient gender Female
Male
Preaquisition Patient age 0-5 years
6-10 years
11-15 years
16-20 years
21-25 years
26-30 years
31-35 years
36-40 years
41-45 years
46-50 years
51-55 years
56-60 years
61-65 years
66-70 years
71-75 years
75-80 years
81-85 years
86-90 years
90-95 years
96-100 years
Storage Time at room temperature 0 h
2 h
4 h
6 h
8 h
10 h
12 h
14 h
16 h
18 h
20 h
22 h
24 h
28 h
32 h
36 h
40 h
44 h
48 h