Lack of consistency between two commercial ELISAs and against an in-house ELISA for the detection of CD36 in human plasma.

Author(s): Lykkeboe S, Larsen AL, Handberg A

Publication: Clin Chem Lab Med, 2012, Vol. 50, Page 1071-4

PubMed ID: 22706248 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of centrifugation speed (1000 x g versus 1850 x g), freeze-thaw cycling, and ELISA manufacturer on the quantification of CD36 in EDTA-plasma. The in-house protocol required 3 freeze-thaw cycles.

   Summary of Findings:

   CD36 levels in specimens centrifuged at 1000 x g and 1850 x g were very strongly correlated when measured by the Adipobioscience ELISA (r=0.99, p<0.001) and strongly correlated using the Cusabio Biotech (r=0.80, p=0.006) and in-house ELISAs (r=0.85, p=0.002). Further, CD36 levels in specimens subjected to 3 freeze-thaw cycles (in house ELISA) and those subjected to none were very strongly correlated when measured by the Adipobioscience ELISA (r=0.91, p<0.001) and strongly correlated using the Cusabio Biotech ELISA (r=0.81, p=0.004). However, CD36 levels determined by the three different ELISAs were not correlated with each other and differed by 2 to more than 1000 fold. Cusabio Biotech ELISA values were at the low end of the assay sensitivity making dilution not possible. The Adipobioscience ELISA had a higher than optimal background.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   ELISA Specific	Technology platform	Cusabio Biotech Adipobioscience In house
   Storage	Freeze/thaw cycling	0 cycles 3 cycles
   Biospecimen Aliquots and Components	Centrifugation	Multiple speeds compared

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