Haematological and iron metabolism parameters in professional cyclists during the Giro d'Italia 3-weeks stage race.
Author(s): Corsetti R, Lombardi G, Lanteri P, Colombini A, Graziani R, Banfi G
Publication: Clin Chem Lab Med, 2012, Vol. 50, Page 949-56
PubMed ID: 22628339 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of strenuous exercise on hematology and clinical chemistry analytes among specimens from professional cyclists competing in a 3 week stage race. Blood specimens were kept at 4 degrees C for up to 8 hours during transportation by train and car to the laboratory. K2EDTA-anticoagulated blood was homogenized and hematology analytes were measured. Blood collected in plain tubes was centrifuged, and serum was stored at -80 degrees C until clinical chemistry analysis.
Summary of Findings:
PV was significantly higher in specimens taken on day 12 or 22 of the race than on day -1. Ht, Hb and RBC counts were lower in specimens taken on day 12 than those from day -1, but they did not decrease further during the last half of the race. Ret% was not affected by the strenuous exercise. However, the IRF was higher in specimens taken on day 12 than those taken on day -1, but IRF returned to baseline levels in day 22 specimens. PLT, WBC counts, MCV, MCH, MCHC, and RDW were not significantly different between specimens taken on different days during the race. When serum levels were corrected for the changes observed in PV, significant decreases in Fe and Tf concentrations were observed between specimens taken on day -1 and day 12 or 22 of the race, but for both analytes, levels were lower on day 12 than on day 22. Ferritin and sTfR levels were not significantly different between specimens taken on different days of the race. Hp levels were significantly higher in specimens taken on day 12 or 22 compared to those taken on day -1 after one outlier was removed from the statistical analysis. CRP levels showed a non-significant increasing trend throughout the race when corrected for PV changes.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Protein Hematology/ auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Time of biospecimen collection Day -1
Day 12
Day 22