Comparison between capillary, venous and arterial levels of protein S100B in patients with severe brain pathology.

Author(s): Astrand R, Romner B, Reinstrup P, Friis-Hansen L, Undén J

Publication: Clin Chem Lab Med, 2012, Vol. 50, Page 1055-61

PubMed ID: 22706246 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of sampling location (capillary, venous, and arterial blood) on protein S100B measurements in blood from patients with severe brain pathology. Some specimens were collected in Sweden and analyzed fresh, and additional specimens were collected in Denmark and analyzed after freezing serum at -80 degrees C prior to analysis. Venous, capillary, and arterial blood specimens were collected simultaneously.

   Summary of Findings:

   There were no significant differences in S100B measurements between capillary specimens obtained simultaneously from different fingers. The authors identified two potential outlier cases where venous specimens had significantly elevated S100B compared to levels in the simultaneous arterial and capillary collections and present statistical evaluation both with and without these outliers. With the exclusion of the two potential outliers, capillary S100B levels were slightly higher than venous S100B levels (0.076 ug/L, p<0.001), and venous S100B levels were slightly higher than arterial S100B levels (0.013 ug/L). Capillary S100B levels were on average 0.095 ug/L higher than arterial S100B levels. Very strong correlations were observed between average S100B levels in capillary and venous specimens (R=0.974), venous and arterial specimens (R=0.944), and capillary and arterial specimens (R=0.984).

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen
   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Severe brain pathology
   Biospecimen Acquisition	Anatomical location of blood draw	Artery Capillary Vein First finger Second finger

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