Stability of serum samples and hemolysis interference on the high sensitivity troponin T assay.

Author(s): Li A, Brattsand G

Publication: Clin Chem Lab Med, 2011, Vol. 49, Page 335-6

PubMed ID: 21077790 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of hemolysis and storage of blood specimens at room temperature on serum troponin T levels as measured by the hs assay. Hemolysis free serum pools with troponin T were added to blood for non-hemolysed specimens, and hemolysate was added to serum specimens containing troponin T to resemble hemolysed specimens.

   Summary of Findings:

   Storage of blood specimens at room temperature had no significant effect on serum troponin T levels in the absence of hemolysis. However, in the presence of hemolysis, storage at room temperature resulted in negative interference with the troponin T hs assay in a hemoglobin concentration-dependent and time-dependent, but troponin T concentration-independent manner. However, storage of hemolysed specimens at room temperature for up to 6 hours resulted in changes in troponin T measurements of less than 10%, except at the highest degree of hemolysis (2 g/L of added hemoglobin).

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	15 min 0 h 4 h 6 h 8 h 24 h
   Biospecimen Acquisition	Type of collection container/solution	SST II Advance tubes
   Biospecimen Aliquots and Components	Biospecimen components	35 ng/L troponin T 43 ng/L troponin T 80 ng/L troponin T 480 ng/L troponin T 0 g/L hemoglobin 0-0.12 g/L hemoglobin 0.5 g/L hemoglobin 1 g/L hemoglobin 2 g/L hemoglobin
   Biospecimen Aliquots and Components	Hemolysis	Hemolysate added No hemolysate added Freeze/thaw-induced
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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