Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

Search BRD Papers

Perchlorate (Irenat®) may falsely lower measured ionised calcium.

Author(s): Gruber M, Nehring C, Creutzenberg M, Graf B, Hopf S

Publication: Clin Chem Lab Med, 2011, Vol. 49, Page 1019-24

PubMed ID: 21428857 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare ionized calcium measurements between 5 blood gas analyzers in blood spiked with calcium chloride and/or different concentrations of perchlorate. In addition, the stability of perchlorate in blood stored at room temperature was examined.

   Summary of Findings:

   The measurement of native ionized calcium concentration was unaffected by the addition of perchlorate to blood specimens using the GEM 4000 and cobas b221 blood gas analyzers. However, the Rapidlab 865 and Rapidlab 405 analyzers measured significantly reduced amounts of ionized calcium after specimens were spiked with perchlorate, and the ABL 815 flex measured significantly reduced amounts of ionized calcium when the spiked perchlorate concentrations were above 0.5 mmol/L. When specimens were spiked with additional ionized calcium, but not perchlorate, both the Rapidlab 865 and ABL 815 flex were able to detect hypercalcaemia, but with a 1.4 mmol/L spiked perchlorate concentration, Rapidlab 865 failed to detect hypercalcaemia and ionized calcium measurements measured by ABL 815 flex decreased. The authors state that spiked perchlorate was stable in anticoagulated blood specimens at room temperature for 48 h and that laboratory prepared stock solutions of sodium perchlorate and Irenat sodium perchlorate had identical effects on ionized calcium readings using the Rapidlab 865 and the ABL 815 flex.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Small molecule	Ion selective electrode

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Clinical chemistry/auto analyzer Specific	Technology platform	Cobas b221 GEM 4000 Rapidlab 865 Rapidlab 405 ABL 815 flex
   Storage	Time at room temperature	0 h 24 h 48 h
   Biospecimen Aliquots and Components	Biospecimen components	Spiked sodium perchlorate (Irenat) Spiked calcium chloride Spiked sodium perchlorate (laboratory prepared) Multiple concentrations evaluated

   View more