Lupus anticoagulant testing: analyzing fresh samples after a single centrifugation and after a 6-8 h delay.
Author(s): Froom P, Barak M
Publication: Clin Chem Lab Med, 2011, Vol. 50, Page 367-370
PubMed ID: 22040237 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of a second centrifugation step and storage of plasma on lupus anticoagulant testing.
Conclusion of Paper
A second centrifugation step did not change the dilute Russell viper venom test (dRVVT) or silica clotting time (SCT) outcomes when plasma was undergoing lupus anticoagulant testing. Further, the dRVVT and SCT results were not significantly changed when testing was performed 6-8 h after specimen collection rather than less than 4 h after.
Studies
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Study Purpose
The purpose of this study was to determine the effects of a second centrifugation step and storage of plasma on lupus anticoagulant testing. The temperature used during storage was not reported.
Summary of Findings:
A second centrifugation step did not change the dRVVT or SCT results for plasma. Further the dRVVT and SCT results were not significantly changed when lupus anticoagulant testing was performed 6-8 h after specimen collection rather than less than 4 h after..
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Lupus
- Normal
Platform:
Analyte Technology Platform Morphology Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration <4 h
6-8 h
Hematology/ auto analyzer Specific Technology platform dRVVT
SCT
Biospecimen Aliquots and Components Centrifugation Different number of centrifugation steps compared