Factors reducing hemolysis rates in blood samples from the emergency department.
Author(s): Munnix IC, Schellart M, Gorissen C, Kleinveld HA
Publication: Clin Chem Lab Med, 2011, Vol. 49, Page 157-8
PubMed ID: 20961194 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare hemolysis rates in blood specimens collected through IV catheter with those collected through venipuncture. Needle size, sequential collection, type of catheter, puncture site, and difficulty in catheter placement were also evaluated.
Summary of Findings:
Hemolysis occurred in specimens from 16 out of 100 patients when blood was collected via IV catheter. In the 16 patients who had hemolyzed specimens, hemolysis was primarily noted in the 1st of 4 consecutively collected specimens (100%) while only 25% of the second collections showed hemolysis. IV placement on the hand was a major cause of hemolysis. Difficult catheter placement and use of a direct draw adapter also were associated with increased risk of hemolysis. Interestingly, needle gauge had no effects on hemolysis in this study. No hemolysis was observed in specimens from 50 patients when blood was collected through venipuncture.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Aliquot sequential collection 1st collection
2nd collection
3rd collection
4th collection
Biospecimen Acquisition Needle gauge 18 gauge
20 gauge
Biospecimen Acquisition Cannulation Difficult
Not difficult
Direct draw adapter
Eclipse needle with pre-attached holder
Biospecimen Acquisition Method of fluid acquisition IV catheter
Venipuncture
Biospecimen Acquisition Anatomical location of blood draw Anticubital vein
Forearm
Hand
Left and right sides compared