Effect of storage conditions and freeze/thaw cycles on aquaporin-4 antibody (NMO-IgG) serum levels.
Author(s): Jarius S, Wildemann B
Publication: Clin Chem Lab Med, 2011, Vol. 49, Page 2121-2
PubMed ID: 21936610 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of freeze-thaw cycling and storage temperature on aquaporin-4 antibody levels.
Conclusion of Paper
Aquaporin-4 antibody levels were comparable after serum was stored for 8 days at room temperature or 4 degrees C, 6 days at -80 degrees C followed by 2 days at room temperature, or 8 days at -80 degrees C with 1, 2, 3, or 5 freeze-thaw cycles.
Studies
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Study Purpose
The purpose of this study was to determine the effects of freeze-thaw cycling and storage temperature on aquaporin-4 antibody levels in serum from a patient with Neuromyelitis optica. Specimen thawing occurred at 4 degrees C for 1 h.
Summary of Findings:
Aquaporin-4 antibody levels were comparable after serum was stored for 8 days at room temperature or 4 degrees C, 6 days at -80 degrees C followed by 2 days at room temperature, or 8 days at -80 degrees C with 1, 2, 3, or 5 freeze-thaw cycles.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Other diagnoses
Platform:
Analyte Technology Platform Protein ELISA Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Neuromyelitis optica
Storage Storage temperature -80 degrees C
4 degrees C
Room temperature
Storage Freeze/thaw cycling 1 cycle
2 cycles
3 cycles
5 cycles
Storage Storage duration 6 days and 2 days
8 days