Effect of cigarette smoking on plasma homocysteine concentrations.

Author(s): Mouhamed DH, Ezzaher A, Neffati F, Douki W, Najjar MF

Publication: Clin Chem Lab Med, 2011, Vol. 49, Page 479-83

PubMed ID: 21143017 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of smoking on plasma homocysteine, folic acid, vitamin B12, thiocyanate and urine cotinine. K3EDTA plasma specimens were stored at -80 degrees C for measurement of homocysteine and -20 degrees C for measurement of folates, vitamin B12, and thiocyanates.

   Summary of Findings:

   Smoking led to significantly increased plasma homocysteine, thiocyanates, and urine cotinine concentrations and decreased plasma vitamin B12, and folic acid concentrations. Folic acid and vitamin B12 concentrations were significantly higher in specimens from subjects who had smoked for less than 5 years compared with those that had smoked for over 20 years. Correlations were found between homocysteine concentration and the duration of smoking or the number of cigarettes/day. Significant positive correlations between homocysteine concentration and plasma thiocyanates and cotininuria were found in specimens from individuals who smoked more than 20 cigarettes/day.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Urine
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Ion selective electrode

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Other drugs	Current smoker Non-smoker Smoked 1-5 y Smoked 5-15 y Smoked 15-20 y Smoked >20 y 5-20 cigarettes/day 21-30 cigarettes/day >30 cigarettes/day
   Storage	Storage temperature	-80 degrees C -20 degrees C

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