Stability of selected serum proteins after long-term storage in the Janus Serum Bank.

Author(s): Gislefoss RE, Grimsrud TK, Mørkrid L

Publication: Clin Chem Lab Med, 2009, Vol. 47, Page 596-603

PubMed ID: 19290843 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to evaluate the effects of storage of serum at -25 degrees C for up to 25 years on select clinical chemistry analytes. Specimens were stored at 4 degrees C for 14-28 h prior to centrifugation and storage at -25 degrees C.

   Summary of Findings:

   Storage of serum for 25 years versus 1 month at -25 degrees C resulted in decreased ALT (-73.4%, p<0.001), CK (-96.2%, p<0.001), insulin c-peptide (-98.7%, p<0.001) and ferritin (-18.5%, p=0.01). In contrast, transferrin increased 8.2% in specimens stored for 25 years compared with those stored for 1 month (p<0.001). Albumin and SHBG also showed a possible decline with storage, but the differences were within those expected based on differing average cohort age (44.6 for 1 month versus 42.1 for 25 year specimens). No effects of storage on aspartate amino transferase, cystatin C, or immunoglobulin E or G were found. In conclusion, long term storage results in altered levels of some serum analytes.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Peptide	Clinical chemistry/auto analyzer
   Glycoprotein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 month 2 years 25 years
   Storage	Storage temperature	-25 degrees C

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