Influence of sample storage duration on serum protein profiles assessed by surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF MS).
Author(s): Gast MC, van Gils CH, Wessels LF, Harris N, Bonfrer JM, Rutgers EJ, Schellens JH, Beijnen JH
Publication: Clin Chem Lab Med, 2009, Vol. 47, Page 694-705
PubMed ID: 19416081 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of patient age, stage of cancer, and long-term serum storage at -30 degrees C on SELDI-TOF MS profiles in serum specimens from breast cancer patients.
Summary of Findings:
14 of 76 spectral peak clusters detected among all specimens differed significantly between specimens in the four different storage length groups. Four of the 14 peak clusters corresponded to N-terminal albumin fragments and showed an increase with storage up to 5 years and then a subsequent decrease. Seven fibrinogen fragments were included in the 14 clusters and showed either a continuous decrease with storage time or remained stable for the first 8 years of storage, after which point they decreased rapidly. Two other peak clusters showing an initial increase and then remaining stable were identified as C3a fragments. And 1 peak cluster was unidentified but increased with storage time at -30 degrees C. None of the significantly affected peak clusters were significantly different between specimens from patients with stage 2A or 2B cancer or between specimens from patients of different ages.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Peptide SELDI-TOF MS Protein SELDI-TOF MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 9-32 months
32-64 months
64-96 months
96-128 months
Preaquisition Patient age Younger than or equal to 49.4 y
49.5-61.8 y
Older than 61.8 y
Preaquisition Prognostic factor Breast carcinoma stage 2A
Breast carcinoma stage 2B