Rapid sample preparation and simultaneous quantitation of prostaglandins and lipoxygenase derived fatty acid metabolites by liquid chromatography-mass spectrometry from small sample volumes.
Author(s): Unterwurzacher I, Koal T, Bonn GK, Weinberger KM, Ramsay SL
Publication: Clin Chem Lab Med, 2008, Vol. 46, Page 1589-97
PubMed ID: 18842110 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of freeze-thaw cycling and room temperature or autosampler post-thaw storage on the measurement of fatty acids and their oxidized metabolites in spiked plasma.
Conclusion of Paper
All analytes measured were stable through 3 freeze-thaw cycles compared to after just 1 freeze-thaw cycle. After sample processing, analytes were stable in samples held in the autosampler for 24 h before analysis, but storage of unprocessed post-thaw specimens at room temperature for 24 h resulted in a dramatic increase in arachidonic acid levels and dramatic decreases in leukotriene D4, prostaglandin D2, and prostaglandin E2.
Studies
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Study Purpose
The purpose of this study was to determine the effects of freeze-thaw cycling and room temperature or autosampler post-thaw storage on the measurement of fatty acids and their oxidized metabolites in 20 uL volumes of spiked plasma.
Summary of Findings:
All analytes measured were stable through 3 freeze-thaw cycles compared to recovery after just 1 freeze-thaw cycle. After sample processing, analytes were stable in samples held in the autosampler for 24 h before analysis, but storage of unprocessed post-thaw specimens at room temperature for 24 h resulted in a dramatic increase in arachidonic acid levels and dramatic decreases in leukotriene D4, prostaglandin D2, and prostaglandin E2, regardless of the initial concentrations.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule LC-MS or LC-MS/MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0 h
24 h
Storage Freeze/thaw cycling 1 cycle
2 cycles
3 cycles
Biospecimen Aliquots and Components Biospecimen components Low concentration of fatty acids
Medium concentration of fatty acids
Storage Storage conditions Post-preparative storage in auto-sampler
Pre-preparative storage on bench