Evaluation of pre-analytical, demographic, behavioural and metabolic variables on fibrinolysis and haemostasis activation markers utilised to assess hypercoagulability.

Author(s): Stegnar M, Cuderman TV, Bozic M

Publication: Clin Chem Lab Med, 2007, Vol. 45, Page 40-6

PubMed ID: 17243913 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the correlation of age, gender, smoking status, body mass index (BMI), blood pressure, cholesterol, and triglyceride levels with fibrinolytic variables (t-PA activity and antigen, plasminogen activator inhibitor-1 (PAI-1) activity and antigen, and euglobulin clot lysis time) and haemostasis activation markers (prothrombin fragment 1 + 2 (F1 + 2), thrombin-antithrombin (TAT) complex, and D-dimer) in healthy individuals. Comparisons were also made between blood specimens collected by a trained and untrained nurse.

   Summary of Findings:

   t-PA antigen was significantly higher in men than women, but there was no effect of smoking status. None of the other fibrinolytic variables or haemostasis activation markers were affected by gender or smoking. However several independent correlations were observed between the fibrinolytic variables and preanalytical metabolic variables. Most significantly, t-PA antigen, PAI activity, and PAI-1 antigen were each associated with BMI and triglyceride levels. t-PA antigen was further influenced by low-density lipoprotein-cholesterol (LDL-C) levels and t-PA activity was significantly associated with high-density lipoprotein-cholesterol (HDL-C) levels. The haemostasis activation markers also showed some significant correlations with preanalytical metabolic and demographic variables. Importantly TAT complex and D-dimer were negatively and positively associated with age, respectively. D-dimer was also significantly influenced by total cholesterol and LDL-C levels, while F1 + 2 was negatively associated with HDL-C levels. Blood specimens collected by a nurse who was untrained in phlebotomy technique showed significantly higher values for all haemostasis activation markers when compared to specimens obtained from the same donors by a trained nurse.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA
   Morphology	Macroscopic observation
   Peptide	ELISA
   Steroid	Colorimetric assay
   Lipid	Colorimetric assay
   Lipoprotein	Colorimetric assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Staff trained/experienced in phlebotomy Staff untrained/inexperienced in phlebotomy
   Preaquisition	Patient age	20-92 y
   Preaquisition	Patient gender	Female Male
   Preaquisition	Other drugs	Smoker Non-smoker
   Preaquisition	Blood pressure	Various values
   Preaquisition	Biomarker level	Total cholesterol HDL-C LDL-C Triglycerides
   Biospecimen Aliquots and Components	Blood and blood products	Serum Platelet-poor plasma

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