Influence of the needle bore size used for collecting venous blood samples on routine clinical chemistry testing.

Author(s): Lippi G, Salvagno GL, Montagnana M, Brocco G, Cesare Guidi G

Publication: Clin Chem Lab Med, 2006, Vol. 44, Page 1009-14

PubMed ID: 16879070 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of needle gauge on 14 common clinical chemistry analytes and free hemoglobin levels in serum. Three sequential venipunctures, on alternating arms and different veins, were used to collect specimens within 5 min.

   Summary of Findings:

   With specimens collected using a 21-gauge needle used as a reference, levels of none of the 14 clinical chemistry analytes or free hemoglobin showed changes greater than the reported analytical quality specifications for desirable bias when specimens were collected using 23- or 25-gauge needles. Lower correlation coefficients were observed between specimens collected using a 21-gauge needle and those collected using a 23- or 25-gauge needle for electrolytes including calcium, chloride, potassium, and sodium.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Protein	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Needle gauge	21-gauge 23-gauge 25-gauge
   Biospecimen Aliquots and Components	Aliquot sequential collection	1st collection 2nd collection 3rd collection

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