Influence of short-term venous stasis on clinical chemistry testing.

Author(s): Lippi G, Salvagno GL, Montagnana M, Brocco G, Guidi GC

Publication: Clin Chem Lab Med, 2005, Vol. 43, Page 869-75

PubMed ID: 16201899 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of venous stasis during blood draw on 12 common clinical chemistry analytes. Three separate venipunctures were performed using three separate veins and alternating arms. The specimen without venous stasis was obtained first, followed by a specimen obtained while the volunteer had standardized pressure applied from a sphygmomanometer for 1 minute, and lastly, a specimen was obtained while the same pressure was applied for 3 minutes. Plasma was frozen at -70 degrees C until analysis.

   Summary of Findings:

   Of the 12 analytes tested, 7 showed significant differences in the specimens obtained after 1 min stasis compared to those obtained with no venous stasis. The average levels of ALT, albumin, calcium, cholesterol, CK, and iron were higher after 1 min stasis, while the average potassium levels were lower compared to specimen collected with no venous stasis. After 3 min stasis during specimen collection, in addition to the 7 analytes mentioned above, average chloride and glucose levels were significantly lower, and average levels of creatinine were significantly higher than levels found in specimens collected with no venous stasis. Urea nitrogen and sodium levels were not significantly affected by the use of a sphygmomanometer, for 1 or 3 min, during specimen collection.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Tourniquet times compared

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