Failure of the PAXgene Blood RNA System to maintain mRNA stability in whole blood.

Author(s): Kågedal B, Lindqvist M, Farnebäck M, Lenner L, Peterson C

Publication: Clin Chem Lab Med, 2005, Vol. 43, Page 1190-2

PubMed ID: 16232084 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and duration on RNA integrity and stability of TPMT in PAXgene-preserved blood specimens.

   Summary of Findings:

   RNA integrity was relatively unaffected by storage at 4 or -20 degrees C but was reduced by storage at room temperature for 3 or 7 days. After 3 days at room temperature, the cyclophilin normalized level of TPMT decreased by 60% or more compared to levels in specimens analyzed within 2 h of collection. After 1 day of storage at 4 or -20 degrees C, the cyclophilin normalized level of TPMT decreased by 40% and 10%, respectively, compared to levels in specimens analyzed 2 h after collection. After the first day of storage at 4 degrees C, levels of cyclophilin normalized TPMT did not decrease further, but at -20 degrees C, levels bottomed out after 3 days of storage with a decrease of 25%.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • PAXgene

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   RNA	Automated electrophoresis/Bioanalyzer
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	Room temperature 4 degrees C -20 degrees C
   Storage	Storage duration	2 h 1 day 3 days 7 days
   Real-time qRT-PCR Specific	Targeted nucleic acid	TPMT Cyclophilin

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