Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice.

Author(s): Ramont L, Thoannes H, Volondat A, Chastang F, Millet MC, Maquart FX

Publication: Clin Chem Lab Med, 2005, Vol. 43, Page 1215-7

PubMed ID: 16232088 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of frozen storage and hemolysis on the measurement of NSE in CSF from patients suspected of having neurodegenerative disease. Aliquots of CSF were placed in polyethelene tubes for storage and recentrifuged before analysis. Hemolysate was prepared by lysing cells with toluene and water and vortexing.

   Summary of Findings:

   NSE levels were 27.6% lower than initial levels when CSF was stored at -20 degrees C for 1 month prior to analysis (p<0.0001). A nonsignificant 5.3% decline in CSF NSE levels was found after 6 months of storage at -80 degrees C compared to levels in fresh specimens. NSE in CSF declined by 19.5% after 8 months of storage at -80 degrees C compared to levels in fresh specimens (p<0.0001). NSE levels in CSF were very strongly correlated with hemolysis index.

   Biospecimens

   • Bodily Fluid - Cerebrospinal Fluid

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	Immunoassay
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-20 degrees C -80 degrees C
   Storage	Storage duration	0 days 1 month 3 months 6 months 8 months 9 months
   Biospecimen Aliquots and Components	Hemolysis	Hemolysate added No hemolysate added
   Biospecimen Aliquots and Components	Biospecimen components	<12 umol/L Hb 12-24.9 umol/L Hb 25-35.9 umol/L Hb 36-47.9 umol/L Hb >48 umol/L Hb
   Preaquisition	Diagnosis/ patient condition	Suspected neurodegenerative disease

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2. Study Purpose

   The purpose of this study was to determine the effects of storage at -80 degrees C and hemolysis on the measurement of NSE in serum from patients suspected of having neuroblastoma or small cell lung cancer. Aliquots of serum were placed in polyethelene tubes for storage and recentrifuged before analysis. Hemolysate was prepared by lysing cells with toluene and water and vortexing.

   Summary of Findings:

   NSE levels were unaffected by storage of serum at -80 degrees C. NSE levels in serum were very strongly correlated with hemolysis index.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Neoplastic - Carcinoma
   • Neoplastic - Other

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 1 month 3 months 6 months 9 months
   Biospecimen Aliquots and Components	Hemolysis	Hemolysate added No hemolysate added
   Biospecimen Aliquots and Components	Biospecimen components	<12 umol/L Hb 12-24.9 umol/L Hb 25-35.9 umol/L Hb 36-47.9 umol/L Hb >48 umol/L Hb

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