NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice.

Author(s): Ramont L, Thoannes H, Volondat A, Chastang F, Millet MC, Maquart FX

Publication: Clin Chem Lab Med, 2005, Vol. 43, Page 1215-7

PubMed ID: 16232088 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of frozen storage temperature and duration and hemolysis on the levels of neuron specific enolase (NSE) in serum and cerebrospinal fluid (CSF).

Conclusion of Paper

NSE levels were significantly lower than levels measured in fresh specimens when CSF was stored at -20 degrees C for 1 month or at -80 degrees C for 8 months prior to analysis, but NSE levels were unaffected by storage of serum at -80 degrees C for up to 9 months. NSE levels in serum and CSF were very strongly correlated with the hemolysis index.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of frozen storage and hemolysis on the measurement of NSE in CSF from patients suspected of having neurodegenerative disease. Aliquots of CSF were placed in polyethelene tubes for storage and recentrifuged before analysis. Hemolysate was prepared by lysing cells with toluene and water and vortexing.

    Summary of Findings:

    NSE levels were 27.6% lower than initial levels when CSF was stored at -20 degrees C for 1 month prior to analysis (p<0.0001). A nonsignificant 5.3% decline in CSF NSE levels was found after 6 months of storage at -80 degrees C compared to levels in fresh specimens. NSE in CSF declined by 19.5% after 8 months of storage at -80 degrees C compared to levels in fresh specimens (p<0.0001). NSE levels in CSF were very strongly correlated with hemolysis index.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Not specified
    • Other diagnoses
    Platform:
    AnalyteTechnology Platform
    Protein Immunoassay
    Protein Hematology/ auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Storage temperature -20 degrees C
    -80 degrees C
    Storage Storage duration 0 days
    1 month
    3 months
    6 months
    8 months
    9 months
    Biospecimen Aliquots and Components Hemolysis Hemolysate added
    No hemolysate added
    Biospecimen Aliquots and Components Biospecimen components <12 umol/L Hb
    12-24.9 umol/L Hb
    25-35.9 umol/L Hb
    36-47.9 umol/L Hb
    >48 umol/L Hb
    Preaquisition Diagnosis/ patient condition Suspected neurodegenerative disease
  2. Study Purpose

    The purpose of this study was to determine the effects of storage at -80 degrees C and hemolysis on the measurement of NSE in serum from patients suspected of having neuroblastoma or small cell lung cancer. Aliquots of serum were placed in polyethelene tubes for storage and recentrifuged before analysis. Hemolysate was prepared by lysing cells with toluene and water and vortexing.

    Summary of Findings:

    NSE levels were unaffected by storage of serum at -80 degrees C. NSE levels in serum were very strongly correlated with hemolysis index.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Neoplastic - Carcinoma
    • Neoplastic - Other
    Platform:
    AnalyteTechnology Platform
    Protein Hematology/ auto analyzer
    Protein Immunoassay
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Storage duration 0 days
    1 month
    3 months
    6 months
    9 months
    Biospecimen Aliquots and Components Hemolysis Hemolysate added
    No hemolysate added
    Biospecimen Aliquots and Components Biospecimen components <12 umol/L Hb
    12-24.9 umol/L Hb
    25-35.9 umol/L Hb
    36-47.9 umol/L Hb
    >48 umol/L Hb

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