Controlled storage conditions prolong stability of biochemical components in whole blood.
Author(s): Stahl M, Brandslund I
Publication: Clin Chem Lab Med, 2005, Vol. 43, Page 210-5
PubMed ID: 15843219 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of centrifugation at 1200 x g versus 3000 x g on 7 clinical chemistry analytes in plasma. After centrifugation, plasma was refrigerated at 4 degrees C until analysis.
Summary of Findings:
Centrifugation speed had no effects on albumin, calcium, glucose, lactate dehydrogenase, potassium, sodium or total protein in plasma.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Centrifugation Multiple speeds compared
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Study Purpose
The purpose of this study was to determine the effects of delayed centrifugation on 27 clinical chemistry analytes in plasma. After centrifugation, plasma was stored at 4 degrees C until analysis.
Summary of Findings:
Potassium increased with increasing delays to centrifugation at 17 or 20 degrees C and decreased with increasing delays to centrifugation at 23 or 25 degrees C. However, when specimens were held at 20 degrees C, prior to centrifugation, the change in potassium was clinically irrelevant until at least an 8 h delay. Plasma phosphate decreased when blood was stored at 20 degrees C for 8 h or more before centrifugation. No other clinically relevant effects of delayed centrifugation were observed.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Peptide Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
2 h
4 h
6 h
8 h
10 h
12 h
Storage Storage temperature 17 degrees C
20 degrees C
23 degrees C
25 degrees C
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated