Stability of phosphoprotein as a biological marker of tumor signaling.

Author(s): Baker AF, Dragovich T, Ihle NT, Williams R, Fenoglio-Preiser C, Powis G

Publication: Clin Cancer Res, 2005, Vol. 11, Page 4338-40

PubMed ID: 15958615 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the stability of p-Akt in surgically resected esophagus or gastroesophageal junction specimens with an unspecified cold ischemia time and in small biopsies with no cold ischemia time.

   Summary of Findings:

   Immunostaining of p-Akt was variable in biopsy specimens (9 of 13), and absent in surgically resected specimens (0 of 15). The authors hypothesized that p-Akt was being rapidly dephosphorylated during cold ischemia time in surgically resected specimens, however, cold ischemia times for these specimens were unspecified. A surgically resected colon specimen with a combined warm and cold ischemia time of 20 minutes also had no detectable p-Akt immunostaining. The authors conclude that p-Akt should only be evaluated in biopsies that have been rapidly processed and not in postoperative surgical samples.

   Biospecimens

   • Tissue - Esophagus

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Cold ischemia time	Unspecified 0 min Less than 20 min
   Biospecimen Acquisition	Method of tissue acquisition	Biopsy Surgical resection

   View more