NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

The shift from fluoride/oxalate to acid citrate/fluoride blood collection tubes for glucose testing - The impact upon patient results.

Author(s): Norman M, Jones I

Publication: Clin Biochem, 2014, Vol. 47(7-8), Page 683-5

PubMed ID: 24463230 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to compare plasma glucose measurements between specimens collected in fluoride oxalate tubes and those collected in citrate fluoride EDTA tubes.

Conclusion of Paper

A 14% increase in mean plasma glucose measurements was observed after collection tubes were switched from Grenier sodium fluoride/potassium oxalate tubes to Terumo Glycaemia tubes containing citrate buffer, sodium fluoride, and EDTA-Na2. The authors conclude that the citrate tubes have a better stabilizing effect.

Studies

  1. Study Purpose

    The purpose of this study was to compare plasma glucose measurements between specimens collected in fluoride oxalate tubes and those collected in citrate fluoride EDTA tubes. Specimens included in this study were collected between 2007 and 2012 in either a primary care center or the hospital. During 2007, specimens were collected in sodium oxalate/sodium fluoride tubes from Becton Dickinson, which were replaced by Grenier sodium fluoride/potassium oxalate tubes. In September of 2010, the use of Terumo Glycaemia tubes (citrate buffer, sodium fluoride, and EDTA-Na2) was recommended. The authors identify September 2010 to December 2010 as a period of transition where both the Grenier and Terumo tubes were used.

    Summary of Findings:

    A 14% increase in the mean value of plasma glucose measurements was observed after collection tubes were switched from Grenier sodium fluoride/potassium oxalate tubes (January 2007-August 2010) to Terumo Glycaemia tubes containing citrate buffer, sodium fluoride, and EDTA-Na2 (January 2011-December 2012). No comparison is offered between Grenier sodium fluoride/potassium oxalate tubes and sodium oxalate/sodium fluoride tubes from Becton Dickinson which were initially used for collection in 2007.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Carbohydrate Clinical chemistry/auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Acquisition Anticoagulant Sodium fluoride/potassium oxalate
    Sodium fluoride/sodium oxalate
    Citrate/sodium fluoride EDTA
    Biospecimen Acquisition Time of biospecimen collection January 2007-August 2010
    September 2010-December 2010
    January 2011-December 2012

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