Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

Search BRD Papers

High sensitivity C-reactive protein (Hs-CRP) remains highly stable in long-term archived human serum.

Author(s): Doumatey AP, Zhou J, Adeyemo A, Rotimi C

Publication: Clin Biochem, 2014, Vol. 47, Page 315-8

PubMed ID: 24373927 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of long term storage of serum on levels of CRP. Initial analysis of CRP levels in 30 serum specimens from healthy individuals was conducted on the day of collection or following the manufacturers recommendation of after <8 days at 4 degrees C or <3 years at -20 degrees C (average 3 months). Following baseline measurement, all specimens were stored at -80 degrees C and thawed 7-11 years later, at which point they were assayed by the same method, but using newer reagents.

   Summary of Findings:

   CRP levels were nonsignificantly higher in specimens stored for 7-11 years (average 8.7 years) at -80 degrees C than when initially assayed (after 0 days, <8 days at 4 degrees C, or <3 years at -20 degrees C). This continued to be true when specimens were separated by timing of initial assay (0-8 days, 9-80 day, or 80 days-3 years).

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days to <3 years 0-8 days 9-80 days 80 days to 3 years 7-11 years

   View more