Effect of storage and repeated freeze/thaw on (S) vitamin B12.
Author(s): Jee P, Fernandez L, Perkins SL, Brooks SP
Publication: Clin Biochem, 2014, Vol. 47, Page 344
PubMed ID: 25241679 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of frozen storage and freeze-thaw cycling on serum vitamin B12 levels.
Conclusion of Paper
Serum vitamin B12 levels were unaffected by an additional 10 months of frozen storage and a single additional freeze-thaw cycle, but vitamin B12 levels were reduced when serum was subjected to 4 freeze-thaw cycles instead of 1.
Studies
-
Study Purpose
The purpose of this study was to determine the effects of frozen storage and freeze-thaw cycling on serum vitamin B12 levels. 20 serum specimens from the Canadian Health Measures Survey (CHMS) were shipped on dry ice, thawed and analyzed, stored for an additional 10 months at -20°C, and thawed and analyzed again. 27 CHMS specimens were shipped on dry ice, thawed and analyzed once, and then thawed and analyzed 3 additional times during 2-10 months of storage at -20°C.
Summary of Findings:
Serum vitamin B12 levels were unaffected by an additional 10 months of frozen storage and one additional freeze-thaw cycle. However, after 4 freeze-thaw cycles, serum vitamin B12 levels were an average of 23.5 pmol/L lower than after a single freeze-thaw cycle (p<0.001).
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Freeze/thaw cycling 1 cycle
2 cycles
3 cycles
4 cycles
Storage Storage duration 0 months
10 months