NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

The effects of freeze-thaw on beta-trace protein and beta 2-microglobulin assays after long-term sample storage.

Author(s): Juraschek SP, Coresh J, Inker LA, Rynders GP, Eckfeldt JH, Selvin E

Publication: Clin Biochem, 2012, Vol. 45, Page 694-6

PubMed ID: 22425605 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of a second freeze-thaw cycle and 4-6 additional months of frozen storage on levels of beta-2 microglobulin (B2M) and beta trace protein (BTP) in serum.

Conclusion of Paper

BTP levels were slightly lower after 2 freeze-thaw cycles and additional storage than after 1, but the levels were still very strongly correlated between specimens subjected to 1 and 2 freeze-thaw cycles. B2M levels were comparable after 1 and 2 freeze-thaw cycles with the additional freezer storage duration totaling 4-6 months.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of a second freeze-thaw cycle and 4-6 additional months of frozen storage on levels of B2M and BTP in serum. Prior to the first thawing, all serum specimens were frozen for 15-24 years at -70 degrees C.

    Summary of Findings:

    BTP levels were slightly lower after 2 freeze-thaw cycles and 4-6 months additional storage than after 1 (-0.05 mg/L, p=0.006), but the levels were still very strongly correlated between the two sets of specimens (r2=0.94). B2M levels were comparable after 1 and 2 freeze-thaw cycles with the additional freezer storage duration totaling 4-6 months.

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Protein Clinical chemistry/auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Storage duration 15-24 years
    4-6 months additional
    Storage Freeze/thaw cycling 1 cycle
    2 cycles

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