The effects of freeze-thaw on beta-trace protein and beta 2-microglobulin assays after long-term sample storage.
Author(s): Juraschek SP, Coresh J, Inker LA, Rynders GP, Eckfeldt JH, Selvin E
Publication: Clin Biochem, 2012, Vol. 45, Page 694-6
PubMed ID: 22425605 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
The purpose of this study was to determine the effects of a second freeze-thaw cycle and 4-6 additional months of frozen storage on levels of B2M and BTP in serum. Prior to the first thawing, all serum specimens were frozen for 15-24 years at -70 degrees C.
Summary of Findings:
BTP levels were slightly lower after 2 freeze-thaw cycles and 4-6 months additional storage than after 1 (-0.05 mg/L, p=0.006), but the levels were still very strongly correlated between the two sets of specimens (r2=0.94). B2M levels were comparable after 1 and 2 freeze-thaw cycles with the additional freezer storage duration totaling 4-6 months.
- Not specified
Analyte Technology Platform Protein Clinical chemistry/auto analyzer
Classification Pre-analytical Factor Value(s) Storage Storage duration 15-24 years
4-6 months additional
Storage Freeze/thaw cycling 1 cycle