The effects of freeze-thaw on beta-trace protein and beta 2-microglobulin assays after long-term sample storage.

Author(s): Juraschek SP, Coresh J, Inker LA, Rynders GP, Eckfeldt JH, Selvin E

Publication: Clin Biochem, 2012, Vol. 45, Page 694-6

PubMed ID: 22425605 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of a second freeze-thaw cycle and 4-6 additional months of frozen storage on levels of B2M and BTP in serum. Prior to the first thawing, all serum specimens were frozen for 15-24 years at -70 degrees C.

   Summary of Findings:

   BTP levels were slightly lower after 2 freeze-thaw cycles and 4-6 months additional storage than after 1 (-0.05 mg/L, p=0.006), but the levels were still very strongly correlated between the two sets of specimens (r2=0.94). B2M levels were comparable after 1 and 2 freeze-thaw cycles with the additional freezer storage duration totaling 4-6 months.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	15-24 years 4-6 months additional
   Storage	Freeze/thaw cycling	1 cycle 2 cycles

   View more