Modification of the EMIT immunoassay for the measurement of unbound mycophenolic acid in plasma.

Author(s): Rebollo N, Calvo MV, Martín-Suárez A, Domínguez-Gil A

Publication: Clin Biochem, 2011, Vol. 44, Page 260-3

PubMed ID: 20923675 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of specimen dilution, ultrafiltration temperature, volume, and centrifugation speed (1000 x g versus 2000 x g) and duration (10, 20 30, 40, or 60 min) on levels of fMPA in ultrafiltered plasma.

   Summary of Findings:

   The fMPA concentrations corresponded to the expected values when specimens were diluted with MPA-free calibrator 1, but when they were diluted with MPA-free ultrafiltered plasma, fMPA concentrations were higher than expected. The authors report that the effect of dilution can be easily corrected mathematically. Concentrations of fMPA in ultrafilered plasma were not affected by specimen volume, or centrifugation speed or duration, but significantly lower concentrations of fMPA were found when plasma ultrafiltration occurred at 23-26 degrees C instead of 37 degrees C (p<0.05).

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Small molecule	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Post-transplant renal allograft
   Biospecimen Aliquots and Components	Aliquot size/volume	0.5 mL 1 mL
   Biospecimen Aliquots and Components	Biospecimen components	Diluted with MPA-free calibrator 1 Diluted with MPA-free plasma
   Biospecimen Aliquots and Components	Filtration	Ultrafiltration at 37 degrees C Ultrafiltration at 23-26 degrees C
   Biospecimen Aliquots and Components	Centrifugation	Multiple durations compared Multiple speeds compared

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