Lipid emulsion solution: A novel cause of hemolysis in serum and plasma blood samples.

Author(s): Jaben EA, Koch CD, Karon BS

Publication: Clin Biochem, 2011, Vol. 44, Page 254-6

PubMed ID: 21070759 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to investigate the effects of container type, ratio and concentration of lipid emulsion solution and time at room temperature on hemolysis of blood specimens. Blood samples were spiked with different ratios of lipid emulsion solution to TPN before being aliquoted into plasma separator tubes or blood gas syringes.

   Summary of Findings:

   When an equal amount of lipid emulsion solution and TPN were added to the blood specimen at a final concentration of 10%, gross hemolysis (hemoglobin >300 mg/dL), which increased with time at room temperature, was observed in the plasma separator tube specimens. In contrast, moderate hemolysis (150 mg/dL), which was not dependent on time at room temperature, was observed in the blood gas syringe specimens when an equal amount of lipid emulsion solution and TPN were added at a final concentration of 10%. As the ratio of lipid emulsion solution to TPN decreased, the hemolysis became less pronounced, regardless of container, and the effect of time at room temperature disappeared. Only minimal hemolysis was observed when equal amounts of lipid emulsion solution and TPN were added at a concentration of 1%. However at higher concentrations, significant hemolysis was observed in the plasma separator tube specimens, and minimal hemolysis was observed in the blood gas syringe specimens.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 min 10 min 20 min 30 min 60 min
   Biospecimen Acquisition	Type of collection container/solution	Plasma separator tubes Blood gas syringe
   Biospecimen Aliquots and Components	Biospecimen components	10% of 1:1 lipid emulsion solution to TPN 10% of 1:4 lipid emulsion solution to TPN 10% of 1:8 lipid emulsion solution to TPN 1% of 1:1 lipid emulsion solution to TPN 5% of 1:1 lipid emulsion solution to TPN 15% of 1:1 lipid emulsion solution to TPN 20% of 1:1 lipid emulsion solution to TPN 0% lipid emulsion solution

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