Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Analytical interference of quinolone antibiotics and quinine derived drugs on urinary protein determined by reagent strips and the pyrogallol red-molybdate protein assay.

Author(s): da Silva AS, Falkenberg M

Publication: Clin Biochem, 2011, Vol. 44, Page 1000-4

PubMed ID: 21635881 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of quinolone antibiotics and anti-malarial drugs on the measurement of urine protein concentrations by two test strips and the PRM assay.

   Summary of Findings:

   False positives for urinary protein were found using the MultiStix 10 SG when therapeutic or supratherapeutic levels of the antimalarial drugs hydroxychloroquine sulfate and chloroquine diphosphate were added to urine or when supratherapeutic levels of quinine sulfate were added to urine. Supratherapeutic levels (350 mg/dL) of the quinolone antibiotics ofloxacin, ciprofloxacin hydrochloride, and norfloxacin also caused false positives for urine protein when using the MultiStix 10 SG test strips. Using the PRM assay, therapeutic and supratherapeutic levels (35 mg/dL or 350 mg/dL) of levofloxacin, ofloxacin, ciprofloxacin hydrochloride, and norfloxacin resulted in false positives for urine protein. All tested concentrations of the anti-malarial drugs (chloroquine diphosphate, hydroxychloroquine sulfate and quinine sulfate) interfered with urine protein measurement by the PRM assay. There were no false positives for urine protein when the Combur 10 test M was used.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Other drugs	Levofloxacin Ofloxacin Ciprofloxacin hydrochloride Norfloxacin Chloroquine diphosphate Hydroxychloroquine sulfate Quinine sulfate
   Clinical chemistry/auto analyzer Specific	Detection method	Multistix 10 SG Combur 10 test M Pyrogallol red-molybdate assay

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