A sensitive method to quantify human cell-free circulating DNA in blood: Relevance to myocardial infarction screening.
Author(s): Jing RR, Wang HM, Cui M, Fang MK, Qiu XJ, Wu XH, Qi J, Wang YG, Zhang LR, Zhu JH, Ju SQ
Publication: Clin Biochem, 2011, Vol. 44, Page 1074-9
PubMed ID: 21723272 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of blood component, gender, and diagnosis of MI on cf-DNA levels in blood. Blood was separated after collection and then frozen at -80 degrees C until all specimens were obtained. Serum was collected in serum separator tubes (SST).
Summary of Findings:
Serum had more cf-DNA than EDTA plasma. There was insignificantly lower cf-DNA in EDTA plasma from males than females (p>0.05), but males had significantly lower levels of cf-DNA in serum than females (mean 154.6 versus 255.8 ng/mL, P<0.05). The levels of cf-DNA were much higher in the plasma of patients with MI than in normal controls (median of 4439.4 and 117.6 ng/mL, respectively). There was no correlation of cf-DNA levels with CK-MB, cTnI, or MYO. In serially collected specimens, cf-DNA peaked before cTnI. The assay was able to accurately quantify cf-DNA at concentrations of 20-400 ng/mL with a coefficient of variance of 13.04, 7.54, and 7.53 for 20 ng/mL, 100 ng/mL and 200 ng/mL, respectively.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
- Cardiovascular Disease
Platform:
Analyte Technology Platform DNA Branch DNA assay Protein Immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Preaquisition Diagnosis/ patient condition Myocardial infarct
Normal
Preaquisition Patient gender Female
Male
Immunoassay Specific Targeted peptide/protein Troponin 1
Creatine kinase-MB
Myoglobin
Biospecimen Acquisition Time of biospecimen collection At admission
Before angioplasty
After angioplasty