Validation of an automated intact N-terminal propeptide of type I procollagen (PINP) assay.

Author(s): Koivula MK, Richardson J, Leino A, Valleala H, Griffiths K, Barnes A, Konttinen YT, Garrity M, Risteli J

Publication: Clin Biochem, 2010, Vol. 43, Page 1453-7

PubMed ID: 20923676 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage, assay type, and disease on intact and total PINP in serum specimens from control, renal failure and rheumatoid arthritis patients. All patient specimens were stored at -20 degrees C.

   Summary of Findings:

   When serum was stored at room temperature or 4 degrees C, for up to 168 h, no change in intact or total PINP was found, but when stored at 37 degrees C both intact and total PINP began to decrease after 8 h of storage. A correlation of 0.986 was found for intact PINP measured by RIA and autoanalyzer in hemodialysis patients. No linear correlation was found between intact and total PINP measured by autoanalyzer in hemodialysis patients, but correlations of 0.947 before treatment and 0.966 after treatment were found for rheumatoid arthritis patients.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Other diagnoses
   • Normal
   • Rheumatoid Arthritis

   Platform:

   Analyte	Technology Platform
   Peptide	Radioimmunoassay
   Peptide	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	37 degrees C 4 degrees C Room temperature
   Storage	Storage duration	0 h 4 h 8 h 24 h 48 h 72 h 96 h 120 h 144 h 168 h
   Preaquisition	Diagnosis/ patient condition	Renal failure
   Preaquisition	Other drugs	Disease modifying antirheumatic drugs Corticosteroids Bisphosphonates

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