Stability of analytes related to clinical chemistry and bone metabolism in blood specimens after delayed processing.

Author(s): Zwart SR, Wolf M, Rogers A, Rodgers S, Gillman PL, Hitchcox K, Ericson KL, Smith SM

Publication: Clin Biochem, 2009, Vol. 42, Page 907-10

PubMed ID: 19250930 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effect of storage of whole blood without anticoagulant at room temperature in the dark for up to 24 h on clinical chemistry and bone metabolism analytes including 25-hydroxyvitamin D, alanine aminotrasferase, albumin, alkaline phosphatase, aspartate aminotrasferase, alpha 1 globulin, alpha 2 globulin, beta globulin, calcium, bone-specific alkaline phosphatase, ceruloplasmin, chloride, cholesterol, copper, cortisol, creatinine, ferritin, folate, gamma globulin, homocysteine, iron, methylmalonic acid, osteocalcin, PTH, potassium, PLP, retinol-binding protein, selenium, sodium, total protein, transferrin, transferrin receptors, transthyretin, total antioxidant capacity, triglycerides and zinc.

   Summary of Findings:

   Storage of whole blood at room temperature for 24 h prior to centrifugation and aliquoting serum significantly increased homocysteine (55%, p<0.001), zinc (20%, p<0.001), and PLP (10%, p<0.05), but significantly decreased osteocalcin (-30%, p<0.001), and PTH (-15%, p<0.05). The authors state that none of these changes are likely to be clinically significant when taken as a whole since the means remained in the reference range. However, for individual patients, these changes may result in an altered interpretation of risk.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Glycoprotein	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer
   Peptide	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	45 min 2.5 h 5 h 24 h
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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