Influence of sodium fluoride on the stability of human blood samples and blood gas measurements.

Author(s): Licht M

Publication: Clin Biochem, 2005, Vol. 38, Page 79-83

PubMed ID: 15607321 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of NaF concentration and time at room temperature on blood gas analysis. Sodium heparin blood from each of the 6 volunteers was simultaneously distributed using a distributor block between capillary tubes containing different concentrations of sodium fluoride and stored for up to 65 min at room temperature.

   Summary of Findings:

   pH increased and pCO2 decreased with increasing concentrations of NaF (1-4 mg/mL) compared to blood containing no NaF. Storage of specimens at room temperature for up to 65 min had no effects on pH or pCO2, regardless of NaF concentration, but pO2 decreased with storage (p<0.01). The starting pO2 and magnitude of the decrease were lower when the specimen contained 3 or 4 mg/mL NaF as opposed to 1-2 mg/mL NaF.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	pH

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Multiple concentrations evaluated Sodium fluoride Sodium heparin
   Storage	Time at room temperature	1 min 3 min 8 min 11 min 13 min 16 min 18 min 21 min 23 min 26 min 28 min 32 min 57 min 58 min 60 min 63 min 65 min

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