NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Hormone stability in human whole blood.

Author(s): Jane Ellis M, Livesey JH, Evans MJ

Publication: Clin Biochem, 2003, Vol. 36, Page 109-12

PubMed ID: 12633759 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of delaying blood separation for up to 24 h on hormone levels in EDTA-plasma.

Conclusion of Paper

When whole blood was stored at 4 or 24 degrees C, prior to centrifugation, there was a significant decrease in insulin levels (by Elecsys assay) and adrenocorticotrophic hormone (ACTH) levels. Arginine vasopressin (AVP) and vasoactive intestinal polypeptide (VIP) increased significantly when specimens were stored at 24 degrees C, but not 4 degrees C, prior to centrifugation.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of delaying blood separation for up to 24 h on hormone levels in EDTA-plasma. After centrifugation, plasma was stored at less than -15 degrees C until analysis.

    Summary of Findings:

    A significant decrease in plasma ACTH levels occurred with storage of whole blood at 4 or 24 degrees C (both p<0.001), prior to centrifugation. When blood was held at 4 or 24 degrees C prior to centrifugation, insulin levels declined when measured by the Elecsys assay (both p<0.05), but not when measured by the IMX assay, which had a high level of intrapatient variability. AVP and VIP increased significantly when specimens were stored at 24 degrees C (p<0.001 and p<0.05, respectively), but not 4 degrees C, prior to centrifugation. The authors extrapolated that 10% losses of ACTH and insulin (by Elecsys) occurred after 18.6 h and 16.9 h, respectively at 4 degrees C or 17.5 h and 16.8 h, respectively at 24 degrees C. The extrapolated times after which there were 10% gains in AVP and VIP levels were 2.6 and 18.6 h, respectively, at 24 degrees C. Aldosterone, alpa-subunits of gonadotrophin (ASU), c-peptide, estradiol, follicle stimulating hormone (FSH), glucagon, growth hormone, insulin-like growth factor 1 (IGF-1), IGF-binding protein 3 (IGF-BP3), leptin, leutenizing hormone (LH), parathyroid hormone (PTH) and prolactin levels were not affected by storage of whole blood at 4 or 24 degrees C, prior to centrifugation.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Normal
    Platform:
    AnalyteTechnology Platform
    Glycoprotein Clinical chemistry/auto analyzer
    Peptide Clinical chemistry/auto analyzer
    Protein Clinical chemistry/auto analyzer
    Steroid Clinical chemistry/auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Storage temperature 4 degrees C
    24 degrees C
    Storage Storage duration 0.5 h
    6 h
    24 h
    Clinical chemistry/auto analyzer Specific Technology platform IMX assay
    Elecsys assay
    Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated

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