Hormone stability in human whole blood.
Author(s): Jane Ellis M, Livesey JH, Evans MJ
Publication: Clin Biochem, 2003, Vol. 36, Page 109-12
PubMed ID: 12633759 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
The purpose of this study was to determine the effects of delaying blood separation for up to 24 h on hormone levels in EDTA-plasma. After centrifugation, plasma was stored at less than -15 degrees C until analysis.
Summary of Findings:
A significant decrease in plasma ACTH levels occurred with storage of whole blood at 4 or 24 degrees C (both p<0.001), prior to centrifugation. When blood was held at 4 or 24 degrees C prior to centrifugation, insulin levels declined when measured by the Elecsys assay (both p<0.05), but not when measured by the IMX assay, which had a high level of intrapatient variability. AVP and VIP increased significantly when specimens were stored at 24 degrees C (p<0.001 and p<0.05, respectively), but not 4 degrees C, prior to centrifugation. The authors extrapolated that 10% losses of ACTH and insulin (by Elecsys) occurred after 18.6 h and 16.9 h, respectively at 4 degrees C or 17.5 h and 16.8 h, respectively at 24 degrees C. The extrapolated times after which there were 10% gains in AVP and VIP levels were 2.6 and 18.6 h, respectively, at 24 degrees C. Aldosterone, alpa-subunits of gonadotrophin (ASU), c-peptide, estradiol, follicle stimulating hormone (FSH), glucagon, growth hormone, insulin-like growth factor 1 (IGF-1), IGF-binding protein 3 (IGF-BP3), leptin, leutenizing hormone (LH), parathyroid hormone (PTH) and prolactin levels were not affected by storage of whole blood at 4 or 24 degrees C, prior to centrifugation.
- None (Fresh)
Analyte Technology Platform Glycoprotein Clinical chemistry/auto analyzer Peptide Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer
Classification Pre-analytical Factor Value(s) Storage Storage temperature 4 degrees C
24 degrees C
Storage Storage duration 0.5 h
Clinical chemistry/auto analyzer Specific Technology platform IMX assay
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated