Hormone stability in human whole blood.

Author(s): Jane Ellis M, Livesey JH, Evans MJ

Publication: Clin Biochem, 2003, Vol. 36, Page 109-12

PubMed ID: 12633759 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of delaying blood separation for up to 24 h on hormone levels in EDTA-plasma. After centrifugation, plasma was stored at less than -15 degrees C until analysis.

   Summary of Findings:

   A significant decrease in plasma ACTH levels occurred with storage of whole blood at 4 or 24 degrees C (both p<0.001), prior to centrifugation. When blood was held at 4 or 24 degrees C prior to centrifugation, insulin levels declined when measured by the Elecsys assay (both p<0.05), but not when measured by the IMX assay, which had a high level of intrapatient variability. AVP and VIP increased significantly when specimens were stored at 24 degrees C (p<0.001 and p<0.05, respectively), but not 4 degrees C, prior to centrifugation. The authors extrapolated that 10% losses of ACTH and insulin (by Elecsys) occurred after 18.6 h and 16.9 h, respectively at 4 degrees C or 17.5 h and 16.8 h, respectively at 24 degrees C. The extrapolated times after which there were 10% gains in AVP and VIP levels were 2.6 and 18.6 h, respectively, at 24 degrees C. Aldosterone, alpa-subunits of gonadotrophin (ASU), c-peptide, estradiol, follicle stimulating hormone (FSH), glucagon, growth hormone, insulin-like growth factor 1 (IGF-1), IGF-binding protein 3 (IGF-BP3), leptin, leutenizing hormone (LH), parathyroid hormone (PTH) and prolactin levels were not affected by storage of whole blood at 4 or 24 degrees C, prior to centrifugation.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Glycoprotein	Clinical chemistry/auto analyzer
   Peptide	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	4 degrees C 24 degrees C
   Storage	Storage duration	0.5 h 6 h 24 h
   Clinical chemistry/auto analyzer Specific	Technology platform	IMX assay Elecsys assay
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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