Stability of PO2, PCO2, and pH in fresh blood samples stored in a plastic syringe with low heparin in relation to various blood-gas and hematological parameters.

Author(s): Beaulieu M, Lapointe Y, Vinet B

Publication: Clin Biochem, 1999, Vol. 32, Page 101-7

PubMed ID: 10211625 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the stability of PO2, pCO2 and pH in blood specimens and the effects of storage on hematological parameters.

   Summary of Findings:

   PO2 decreased with time when specimens were stored at 22 degrees C (p<0.001), but increased with time when stored on ice (p<0.005). However, a large degree of variability in PO2 was observed between specimens stored at either temperature, and in specimens with an initial PO2 of less than 50 mm mercury, the changes with storage were insignificant. The change in PO2 did not correlate with leukocyte count, platelet count or hemoglobin concentration, but in specimens stored at 22 degrees C, the change in PO2 did correlate with SatHbO2. PCO2 increased with storage at either temperature, regardless of initial levels, while pH decreased. The magnitude of the changes in PO2, PCO2, and pH were much greater in specimens stored at 22 degrees C than in those stored on ice.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	pH
   Cell count/volume	Hematology/ auto analyzer
   Protein	Spectrophotometry
   Gas	Radiometer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 min 15 min 30 min 60 min 120 min
   Storage	Storage temperature	22 degrees C On ice
   Biospecimen Acquisition	Anticoagulant	Heparin
   Storage	Type of storage container	Plastic syringe

   View more