Drug-protein binding interferences caused by the plasticizer TBEP.

Author(s): Devine JE

Publication: Clin Biochem, 1984, Vol. 17, Page 345-7

PubMed ID: 6518648 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of contact with TBEP containing stoppers on apparent concentrations of therapeutic drugs in blood. Donor blood was spiked with lidocaine, theophylline, and quinidine.

   Summary of Findings:

   Storage of blood in contact with TBEP containing stoppers resulted in 11-32% and 10-18% decreases in apparent quinidine and lidocaine concentrations, respectively. There was a larger decrease in specimens spiked with low doses rather than higher doses of quinidine or lidocaine. TBEP also caused a decrease in the percentage of free lidocaine and bound theophylline. When lower than normal levels of AAG were present in the plasma, the effects of TBEP on measured lidocaine concentrations were much smaller.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	Immunoassay
   Small molecule	Clinical chemistry/auto analyzer
   Small molecule	Equilibrium dialysis
   Small molecule	GC- flame ionization
   Glycoprotein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Heparin
   Storage	Time at room temperature	2 h
   Storage	Type of storage container	Glass tube with teflon coated screw cap Red-stoppered (containing TBEP) tube Red-stoppered (no TBEP) tube
   Preaquisition	Biomarker level	0.2 g/L AAG (below normal) 0.48 g/L AAG (normal) 0.62 g/L AAG (normal)

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