Effect of time, temperature and freezing on the stability of immunoreactive LH, FSH, TSH, growth hormone, prolactin and insulin in plasma.
Author(s): Livesey JH, Hodgkinson SC, Roud HR, Donald RA
Publication: Clin Biochem, 1980, Vol. 13, Page 151-5
PubMed ID: 6778631 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of storage and freeze-thaw cycling on hormone levels in plasma and urine.
Conclusion of Paper
Levels of follicle-stimulating hormone (FSH) in urine increased slightly during storage of specimens at 4 degrees C, but no other hormones measured were affected by storage of urine or plasma at 4 degrees C. When plasma or urine specimens were stored at 20 degrees C, growth hormone, prolactin, and insulin levels in plasma decreased significantly over the 8 days of storage, but luteinizing hormone (LH), FSH and thyroid-stimulating hormone (TSH) in plasma and LH and FSH in urine were unaffected by the storage at 20 degrees C. When storage was at 37 degrees C, only TSH in plasma remained unaffected with decreases noted for all analytes except FSH which increased in both urine and plasma. Repeated freeze-thaw cycling of plasma decreased insulin levels but did not affect other analytes.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage at 4, 20 and 37 degrees C and freeze-thaw cycling on LH, FSH, TSH, growth hormone, prolactin and insulin levels in plasma as well as LH and FSH levels in urine specimens. After experimental storage, specimens were transferred to -20 degrees C where they were held until analysis.
Summary of Findings:
FSH levels increased slightly during storage of urine at 4 degrees C, but no other hormones measured were effected by storage of urine or plasma at 4 degrees C. When plasma or urine specimens were stored at 20 degrees C, growth hormone, prolactin, and insulin levels in plasma decreased significantly over the 8 days of storage, but LH, FSH and TSH in plasma and LH and FSH in urine were unaffected. When storage was at 37 degrees C, only TSH in plasma remained unaffected with decreases noted for all analytes except FSH which increased in both urine and plasma. Repeated freeze-thaw cycling of plasma decreased insulin levels but did not affect other analytes.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
- Other diagnoses
Platform:
Analyte Technology Platform Glycoprotein Radioimmunoassay Peptide Radioimmunoassay Protein Radioimmunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -20 degrees C
4 degrees C
20 degrees C
37 degrees C
Storage Storage duration 0 days
1 day
2 days
4 days
5 days
7 days
8 days
Storage Freeze/thaw cycling 0 cycles
1 cycle
2 cycles
3 cycles
4 cycles
5 cycles
Biospecimen Acquisition Biospecimen location Plasma
Urine
Preaquisition Diagnosis/ patient condition Hyperprolactinemia