Diurnal, seasonal, and blood-processing patterns in levels of circulating fibrinogen, fibrin D-dimer, C-reactive protein, tissue plasminogen activator, and von Willebrand factor in a 45-year-old population.
Author(s): Rudnicka AR, Rumley A, Lowe GD, Strachan DP
Publication: Circulation, 2007, Vol. 115, Page 996-1003
PubMed ID: 17296859 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of time of day and month of blood collection, patient gender, transport time, and needle type on markers of coronary artery disease. Citrated blood from non-fasting volunteers was transported to the laboratory at ambient temperatures (collections throughout United Kingdom), centrifuged, and plasma was stored frozen at -70 degrees C until analysis.
Summary of Findings:
Fibrinogen and D-dimer levels were higher, and t-PA and vWF levels were lower in women than in men. Time of collection significantly affected levels of fibrinogen (p=0.03), CRP (p=0.003), t-PA (p<0.0001) and vWF (p<0.0001), but not D-dimer. t-PA decreased throughout the day, vWF and fibrinogen peaked at noon, CRP was highest at 3 PM and 10 PM and D-dimer levels peaked at 2 PM. Month of collection significantly affected fibrinogen (p=0.02), D-dimer (p<0.001), t-PA (p=0.0004) and vWF (p=0.003), but not CRP levels (p=0.82). D-dimer peaked between February and March (p<0.001), t-PA peaked in November (p=0.0004), vWF peaked in May (p=0.003), and fibrinogen and CRP levels were highest during winter months (p=0.02 and p=0.82, respectively). Levels of D-dimer (p=0.04), CRP (p=0.03), t-PA (p<0.0001), and vWF (p=0.0005) and the rate of hemolysis were significantly affected by delays in processing due to transport, but fibrinogen levels were not affected. Using a winged needle for blood collection decreased the incidence of hemolysis (p=0.025) but did not affect levels of the other analytes.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Glycoprotein Hematology/ auto analyzer Peptide ELISA Protein ELISA Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Time of biospecimen collection 9 AM
10 AM
11 AM
12 PM
1 PM
2 PM
3 PM
4 PM
5 PM
6 PM
7 PM
8 PM
9 PM
10 PM
January
February
March
April
May
June
July
August
September
October
November
December
Biospecimen Acquisition Method of fluid acquisition Butterfly device
Needle
Preaquisition Patient gender Female
Male
Storage Between site transportation method Unspecified transport method
Storage Specimen transport duration/condition <1 day
2 days
3 days
4 days or more
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated