Morning hypercoagulability and hypofibrinolysis. Diurnal variations in circulating activated factor VII, prothrombin fragment F1+2, and plasmin-plasmin inhibitor complex.

Author(s): Kapiotis S, Jilma B, Quehenberger P, Ruzicka K, Handler S, Speiser W

Publication: Circulation, 1997, Vol. 96, Page 19-21

PubMed ID: 9236409 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of time of specimen collection on FVIIa, FVII antigen, F1+2, active PAI-1 antigen, and PPI levels in plasma, and cholesterol and triglycerides in serum. FVIIa was analyzed immediately while plasma specimens were stored for up to 4 weeks at -80 degrees C prior to analysis of other analytes.

   Summary of Findings:

   On average, FVIIa, F1+2, and active PAI-1 antigen levels decreased by 39%, 19%, and 46%, respectively, between the 8 am and 8pm collection (all P=0.005). PPI levels were 105% higher in specimens collected at 8 pm compared to those collected at 8 am (P=0.008). Serum cholesterol was slightly higher at 8 pm than 8 am (average 3% increase, P=0.012). FVII antigen (plasma) and triglyceride levels in serum were not significantly affected by the time of specimen collection.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer
   Protein	ELISA
   Steroid	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	8:00 am 12:00 pm 4:00 pm 8:00 pm
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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