Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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The effect of venous occlusion on serum cholesterol and total protein concentration-a warning.

Author(s): Page I, MOINUDDIN M

Publication: Circulation, 1962, Vol. 25, Page 651-2

PubMed ID: 14483157 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of tourniquet time on total cholesterol and total protein levels in serum. A blood pressure cuff inflated to 100 mm mercury (Hg) was used for venous occlusion for 2, 5, or 10 minutes before blood specimens were obtained. Control specimens were either obtained with no venous occlusion at the same 2, 5, and 10 min time points (1 volunteer), or with venous occlusion while a needle was introduced, but no venous occlusion for 10 min prior to the specimen being drawn (12 volunteers).

   Summary of Findings:

   Total cholesterol and total protein levels were not significantly different between the 2, 5, and 10 min collection time points when no tourniquet was used. However, when a tourniquet was used during collection, significantly higher concentrations of protein and cholesterol levels were measured in serum after 5 and 10 min tourniquet times, but not after a 2 min tourniquet time, compared to specimens obtained with no tourniquet.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Steroid	Colorimetric assay
   Protein	Colorimetric assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Tourniquet times compared
   Biospecimen Acquisition	Time of biospecimen collection	0 min 2 min 5 min 10 min

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