Circadian variations in blood coagulation parameters, alpha-antitrypsin antigen and platelet aggregation and retention in clinically healthy subjects.

Author(s): Haus E, Cusulos M, Sackett-Lundeen L, Swoyer J

Publication: Chronobiol Int, 1990, Vol. 7, Page 203-16

PubMed ID: 2125246 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of circadian rhythm on coagulation parameters, platelet adhesion and aggregation, and other hematology analytes. Complete blood counts were determined, and plasma cortisol was measured as reference functions. Blood was collected from subjects in the recumbent position at all time points, but during normal waking hours, subjects were ambulatory until 30 min prior to each blood draw.

   Summary of Findings:

   Circadian rhythm was found to affect platelet aggregation in response to ADP and adrenalin, platelet adhesiveness, PT, APTT, TT, fibrinogen, Factor VIII activity, and alpha-1-antitrypsin antigen. None of the changes observed due to time of biospecimen collection were large enough to be of clinical significance, but they were generally suggestive of a state of hypercoagulability during the morning. However, maximal aggregation, in response to ADP or adrenalin, was achieved in platelet rich plasma from specimens collected at 12:00 am. No significant trends could be determined based on the time of biospecimen collection for the levels of plasminogen, alpha-2-macroglobulin, antithrombin III antigen, Factor V, or fibrinogen degradation products due to high degrees of variability.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer
   Morphology	Hematology/ auto analyzer
   Cell count/volume	Light microscopy
   Cell count/volume	Coulter counter
   Steroid	Radioimmunoassay
   Glycoprotein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	8:00 am 12:00 pm 4:00 pm 8:00 pm 12:00 am 4:00 am

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