Impact of evacuated collection tube fill volume and mixing on routine coagulation testing using 2.5-ml (pediatric) tubes.

Author(s): Chuang J, Sadler MA, Witt DM

Publication: Chest, 2004, Vol. 126, Page 1262-6

PubMed ID: 15486391 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of specimen inversion, time prior to specimen mixing, and tube fill volume on INR results derived from PT in specimens from patients on warfarin anticoagulation therapy and healthy volunteers. Plasma was frozen at -20 degrees C until analysis.

   Summary of Findings:

   Neither the number of tube inversions after specimen collection (1-5) nor the duration of time before mixing (0-4 min) had significant effects on mean INR in healthy volunteers or patients receiving warfarin. However, fill volumes of <90% lead to significantly higher INRs compared to tubes that were 100% full for specimens from healthy volunteers (after elimination of 3 outliers) and patients on warfarin (p<0.01 and p<0.001, respectively).

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Cardiovascular Disease
   • Normal
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Other drugs	Warfarin Aspirin None
   Biospecimen Aliquots and Components	Biospecimen mixing	Inverted once Inverted twice Inverted three times Inverted four times Inverted five times After 0 min After 1 min After 2 min After 3 min After 4 min
   Biospecimen Aliquots and Components	Aliquot size/volume	100% full 90% full 80% full 70% full 60% full 50% full
   Preaquisition	Diagnosis/ patient condition	Stroke/cerebrovascular accident

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