The interference of cold ischemia time in the quality of total RNA from frozen tumor samples.

Author(s): Viana CR, Neto CS, Kerr LM, Palmero EI, Marques MM, Colaiacovo T, de Queiroz Junior AF, Carvalho AL, Siqueira SA

Publication: Cell Tissue Bank, 2013, Vol. 14, Page 167-73

PubMed ID: 22562477 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to assess RNA degradation via RIN in thyroid, colorectal, breast, lung, and stomach specimens collected within 30 minutes of excision or after 45 min at room temperature. A total of 98 and 100 tumor specimens were collected for each cold ischemia timepoint (<30 min and 45 min, respectively). Specimens were snap-frozen in liquid nitrogen vapor and stored at -80 degrees C prior to analysis. Specific cold ischemia times were not provided.

   Summary of Findings:

   The percentage of specimens with intact RNA (RIN > 5) was significantly lower after 45 min of room temperature cold ischemia compared to those frozen within 30 min of collection (18% vs 32%; p<0.001). The magnitude of the decline in RIN that was attributed to cold ischemia time was tissue-specific, with the greatest decline in colorectal and thyroid specimens and the smallest changes observed in lung. It is worthy to note that lung also had the lowest percentage of intact RNA at 30 min.

   Biospecimens

   • Tissue - Colorectal
   • Tissue - Stomach
   • Tissue - Lung
   • Tissue - Thyroid Gland
   • Tissue - Breast

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	Automated electrophoresis/Bioanalyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Cold ischemia time	<30 min 45 min
   Biospecimen Acquisition	Biospecimen location	Thyroid Colorectal Breast Lung Stomach

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