The use of in situ hybridization histochemistry for the study of neuropeptide gene expression in the human brain.

Author(s): Mengod G, Charli JL, Palacios JM

Publication: Cell Mol Neurobiol, 1990, Vol. 10, Page 113-26

PubMed ID: 2334944 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to examine the effect of PMI (5.5-15 h), patient age (42-82 y), gender and Huntington's or Parkinson's disease on the levels of neuropeptide transcripts by ISH and northern blot. Specimens from 12 control and 6 Huntington's disease patients were used for analysis.

   Summary of Findings:

   No significant effects of patient age, gender or PMI on the optical density of the ISH signal from preproenkephalin A, somatostatin or neuropeptide Y was observed in the three regions of striatum. Striatum allowed for observation of preproenkephalin A, somatostatin, neuropeptide Y, but not cholecystokinin, arginine-vasopressin and oxytocin. In the hypothalamus, preproenkephalin A, somatostatin, oxytocin and arginine- vasopressin were observed. Huntington's and Parkinson's disease resulted in decreased preproenkephalin A, but not somatostatin or neuropeptide Y. The authors conclude that neuropeptide transcript levels may be examined by ISH or northern blot in postmortem tissue even after a PMI of 15 h and while brain region and disease specific differences occur there was no effect of gender or age.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Cardiovascular Disease
   • Huntington's Disease
   • Parkinson's Disease
   • Autopsy

   Platform:

   Analyte	Technology Platform
   RNA	In situ hybridization
   RNA	Northern blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Postmortem interval	5.5 h 6.5 h 8.5 h 14.5 h 15 h
   Preaquisition	Patient age	42 y 62 y 77 y 82 y
   Preaquisition	Patient gender	Female Male
   Biospecimen Acquisition	Biospecimen location	Striatum-caudate Striatum-putamen Striatum-accumbens Hypothalamus
   In situ hybridization Specific	Targeted nucleic acid	Preproenkephalin A Somatostatin Neuropeptide Y
   Preaquisition	Diagnosis/ patient condition	Huntington's disease Parkinson's disease No neuropsychiatric disorder

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