Analysis of DNA sequences in forty-year-old paraffin-embedded thin-tissue sections: a bridge between molecular biology and classical histology.

Author(s): Shibata D, Martin WJ, Arnheim N

Publication: Cancer Res, 1988, Vol. 48, Page 4564-6

PubMed ID: 2840197 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare PCR amplification of the human papillomavirus (HPV) E6 gene and beta-globin gene fragments among DNA extracted from FFPE biopsy and autopsy specimens stored as paraffin blocks at ambient temperature from less than a year to 40 years.

   Summary of Findings:

   PCR amplification of beta-globin and HPV E6 gene fragments was successful using DNA extracted from FFPE specimens procured at biopsy or autopsy within a year of analysis. PCR amplification of a beta-globin fragment was successful for all archival specimens analyzed, although amplicon abundance displayed significant variability. Amplification of HPV E6 gene fragments was successful in 40% of archived specimens examined.

   Biospecimens

   • Tissue - Cervix

   Preservative Types

   • Formalin

   Diagnoses:

   • Autopsy
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	PCR
   DNA	Dot blot or slot blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	<1 yr 38 yr 40 yr
   Biospecimen Acquisition	Method of tissue acquisition	Autopsy Biopsy

   View more