Differences in gene and protein expression and the effects of race/ethnicity on breast cancer subtypes.

Author(s): Chavez-Macgregor M, Liu S, De Melo-Gagliato D, Chen H, Do KA, Pusztai L, Fraser Symmans W, Nair L, Hortobagyi GN, Mills GB, Meric-Bernstam F, Gonzalez-Angulo AM

Publication: Cancer Epidemiol Biomarkers Prev, 2014, Vol. 23, Page 316-23

PubMed ID: 24296856 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of patient ethnicity on the protein and transcript expression profiles by microarray in FNA breast tumor specimens. For microarray analysis, RNA was isolated from 376 FNA specimens and stored frozen at -80 degrees C until analysis using the affymetrix U133A gene chip. Reverse phase protein arrays were conducted to evaluate the levels of 177 proteins using 255 frozen (-80 degrees C) FNA specimens.

   Summary of Findings:

   None of the 177 proteins was differentially expressed between races, but on average, Hispanic patients had more advanced breast cancer than patients of other races (p<0.0001). Using an initial cohort of 62 HER-2 positive tumors, 2 and 384 probes were significantly affected by race in HR-positive and HER-2 positive specimens, respectively, but these results were not duplicated using a second cohort of 59 specimens.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Frozen
   • None (Fresh)

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	DNA microarray
   Protein	Reverse phase protein microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient race	White Hispanic Black Other

   View more