Reliability of serum biomarkers of inflammation from repeated measures in healthy individuals.

Author(s): Navarro SL, Brasky TM, Schwarz Y, Song X, Wang CY, Kristal AR, Kratz M, White E, Lampe JW

Publication: Cancer Epidemiol Biomarkers Prev, 2012, Vol. 21, Page 1167-70

PubMed ID: 22564866 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the degree of short term variability (4-6 months) of hsCRP, IL6, IL8, TNFa, and sTNFR I and II levels in serum from healthy volunteers. Specimens were stored for 3-7 years at -80 degrees C prior to analysis. The average length of time between 4 separate collections was 6 weeks. Not all analytes were measured at 4 time points from each volunteer.

   Summary of Findings:

   Analysis of analyte levels at multiple timepoints several weeks apart showed that ICCs were very strong for TNFa (0.92) and sTNFR I and II (0.92 and 0.90, respectively). ICCs were also strong for IL-8 and hsCRP (0.73 and 0.62, respectively), but the ICC for IL-6 was modest (0.48).

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Protein	ELISA
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	Collection 1 Collection 2 Collection 3 Collection 4

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