Collection of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort: comparison of the response rate and quality of genomic DNA.

Author(s): Hansen TV, Simonsen MK, Nielsen FC, Hundrup YA

Publication: Cancer Epidemiol Biomarkers Prev, 2007, Vol. 16, Page 2072-6

PubMed ID: 17932355 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare DNA quantity and quality among self-collected saliva and buccal cell specimens (swab, FTA card) with clinic-collected blood specimens.

   Summary of Findings:

   DNA quantity was greatest among buccal cell specimens collected using mouth swabs followed by saliva specimens and clinic-collected blood specimens, with buccal cells collected via FTA card generating the lowest yield. DNA quality, determined by 260/280 OD ratio, was highest among blood specimens followed by saliva, and buccal cells collected via mouth swab and FTA card. Satisfactory results obtained for both genotyping and qPCR analysis was limited to blood and saliva specimens.

   Biospecimens

   • Bodily Fluid - Saliva
   • Cell - Buccal
   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Spectrophotometry
   DNA	Electrophoresis
   DNA	Real-time qPCR
   DNA	DNA sequencing

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Venipuncture Expectoration
   Biospecimen Acquisition	Biospecimen location	Blood Saliva Buccal cell
   Biospecimen Acquisition	Method of cell acquisition	Mouth swab FTA card

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