Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

Search BRD Papers

Quality and quantity of saliva DNA obtained from the self-administrated oragene method--a pilot study on the cohort of Swedish men.

Author(s): Rylander-Rudqvist T, Håkansson N, Tybring G, Wolk A

Publication: Cancer Epidemiol Biomarkers Prev, 2006, Vol. 15, Page 1742-5

PubMed ID: 16985039 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study evaluated DNA quality and quantity as well as genotyping success rates of DNA extracted from patient self-collected saliva specimens using the Oragene saliva collection method. Of note, controls were not performed.

   Summary of Findings:

   Self-collected saliva specimens produced a mean DNA yield of 40-135 ug total DNA (depending upon the technology platform used), which was not correlated to patient age, and a mean A260/A280 ratio of 1.76. Real-time qPCR analysis of the prothrombin gene estimated approximately 68% of isolated DNA to be of human origin. High molecular weight DNA (>23 kb) was present in all samples. SNP assay of saliva DNA and whole genome amplified DNA yielded 91-100% and 88-99% success rates, respectively.

   Biospecimens

   • Bodily Fluid - Saliva

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Spectrophotometry
   DNA	Electrophoresis
   DNA	Real-time qPCR
   DNA	SNP assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient age	53-61 y 62-66 y 67-71 y 77-87 y
   SNP assay Specific	Nucleic acid amplification	Whole genome amplification Unamplified DNA

   View more